i-custom oligo process workstation

indawo yokusebenza yenqubo ye-oligo yangokwezifiso,
i-oligo inqubo yokusekela imishini,

Ingxenye ye-Elution

1. Umugqa wezimbobo zomjovo we-liquid eziyi-8, ongajovwa ngasikhathi sinye, futhi uketshezi oluyisihluthulelo lungalawulwa ngakunye.
2. Izikhundla ezi-5 zamabhodlela e-reagent, ibhodlela ngalinye lifakwe ibhodlela le-reagent ye-4L, okungukuthi, lingahambisana nezinhlobo ezi-5 zama-reagents.
3. Ithuluzi liqhutshwa uketshezi olucindezelayo oluhle, olulula futhi oluguquguqukayo ukulawula izinga lokugeleza kwamanzi.
4. Imishini ifakwe indawo yepuleti yemithombo engu-96, futhi uhlobo lwepuleti lucaciswe yikhasimende.
5. Ipuleti elinemithombo ejulile engu-96 lingafakwa ngaphansi kwepuleti ukuze liqoqe uketshezi, noma lingaxhunywa ngokuqondile epayipini ukuze kukhishwe uketshezi olulahlwayo.
6. Engxenyeni yesofthiwe, ungakhetha uhlobo lwe-reagent kanye nenani le-reagent yokwengeza amasampula, isikhathi singasethwa, igiya lokucindezela lingasethwa, isikhathi sokucindezela singalawulwa, futhi izinyathelo zingasethwa ukuze gijimani njalo.2 izinhlobo zokupheka.

Ingxenye yokuhlanza

1. Izinto ezisetshenziswayo zingagcwalisa amakholomu angama-96 c18 ngesikhathi esisodwa.
2. Izinto ezisetshenziswayo ezijwayelekile zifakwe izimbobo ze-reagent ezinhlanu, isibambi seshubhu le-centrifuge, futhi umbambi angathwala umkhawulo wamashubhu we-primer centrifuge angu-96.
3. Izinto zokusebenza zamukela uhlelo lokulawula oluzimele lwe-axis emithathu, ngokunemba okuphezulu nokuphindaphinda okuhle.
4. Isisetshenziswa samukela uhlobo lwesihlungi sokumunca.
5. Izilungiselelo zesofthiwe ziyavumelana nezimo futhi kulula ukusebenza.
6. Ikhanda lokumunca lifakwe idivayisi ezwa izinga loketshezi ekwazi ukuzwa ngokuzenzakalelayo ubude bezinga loketshezi.

Ingxenye yokufaka amapayipi

1. Ipuleti lemithombo engama-96 ukuya kushubhu ye-centrifuge.
2. Ithiphu ye-pipette ezenzakalelayo: i-primer ngayinye ingahlukaniswa ibe yimichilo emi-2 kakhulu.
3. X, Y, Z ukunyakaza kwe-eksisi.
4. Ububanzi bevolumu yokumunca (5-200ul).
5. Lapho uhlukanisa amashubhu amaningi.
6. Izidingo zesikhathi.
7. Ingaxhunyaniswa nesifanekiso sokulinganisa,Umsebenzi wokusheshisa iphutha, kukhona umsebenzi wokumisa isikhashana phakathi Okubomvu okhombisa ukuthi isamba sevolumu ayanele futhi sidinga ukugqagqana.
Kukhona amathiphu abomvu, aphuzi futhi aluhlaza okubala ivolumu: okuphuzi kusho ukuthi ivolumu incane, ungakwazi ukuguqula ivolumu ngesandla, asikho isidingo sokudlulana, okuluhlaza kusho okujwayelekile.
8. Ulwazi olufana nenombolo yekholomu, inombolo yamashubhu, kanye nevolumu yeshubhu ngayinye kuboniswa lapho kugcwaliswa isampula ngayinye.Isimo sibonisa ukuthi ama-primer ahlukanisiwe futhi awahlukaniswanga, ukulungiswa kokumisa okuzenzakalelayo ngaphambi kokukhipha ngakunye.

I-oligo process workstation ye-oligo process aupport equipment, ine-fuction ye-Pepetting, ukuxuba, ukunyakazisa, ukushisisa nokunye.