Izicupho zamangqamuzana ze-phosphoramidite nama-reagents

Isicelo:

I-Molecular Trap isetshenziselwa ukukhangisa amanzi okulandelela kuma-reagents kanye ne-amidite, ekuqaleni yayiklanyelwe ukuhlanganiswa kwe-oligonucleotides.Ilungele, ayinalo uthuli, futhi ayinaflaneli.Ingangezwa ezinhlobonhlobo zezincibilikisi kanye nezixazululo ze-organic ukuze kukhishwe amanani omkhondo wamanzi.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo Yomkhiqizo

Kuhlolo lwe-ABI ne-MilliGen/PerSeptive DNA synthesis, iphakethe le-sub-sieve lingagcina okuqukethwe kwamanzi ku-nitrile namabhodlela e-activator ngaphansi kuka-10 ppm, ngaphandle kokukhathazeka ngokuthi i-valve noma i-throttle valve ihlukaniswa ibe isisefo esincane.Uthuli noma i-lint esikhwameni sokupakisha ivalekile.Ingasetshenziselwa ngokuqondile ukukhishwa kwamanzi ka-500 ml, 1L, 2 L namanye amabhodlela e-solvent, kanye nokucaciswa kwephakethe le-sub-sieve nakho kungenziwa ngezifiso.

Okulandelayo kubonisa umphumela oguquguqukayo wokuqeda amanzi we-10 g yesicupho samangqamuzana ku-500ml, 1L, 4L amasampuli e-nitrile anokuqukethwe kwamanzi okuhlukile.

Izicupho ze-Molecular2

Qhathanisa ne-import molecular trap

500 ml 197 ppm ACN

Isikhathi(h)

0

24

48

72

96

HonyaBio

197

33

16.5

6.5

6

Amanye Amazwe

197

43

27

15

15

1 L 143 ppm ACN

Isikhathi(h)

0

24

48

72

96

HonyaBio

143

48

32

20

15

Amanye Amazwe

142

47

36

23

15

4 L 141 ppm ACN

Isikhathi(h)

0

24

48

72

96

HonyaBio

141

95

94

84

73

Amanye Amazwe

141

96

95

85

72

Imiyalelo kanye nokusetshenziswa

Isicupho samangqamuzana sigcwele i-vacuum, futhi sidinga ukuvulwa ngisho noma sisetshenziswa, futhi uphawu lwebhodlela le-reagent kufanele luqinisekiswe ngesikhathi sokusetshenziswa.
Isisefo se-2g samahora angu-24 singanciphisa okuqukethwe kwamanzi angu-165 ppm ku-500 ml nitrile kuya ku-105 ppm.
Isisefo esingu-5 g samahora angu-24 singanciphisa okuqukethwe kwamanzi angu-172 ppm ku-500 ml nitrile kuya ku-58 ppm.
I-10 g isisefo 24 h Inganciphisa okuqukethwe kwamanzi angu-166 ppm ku-1 L nitrile kuya ku-68 ppm.
I-sub-sieve engu-20 g inganciphisa okuqukethwe kwamanzi angu-162 ppm ku-4 L nitrile kuya ku-109 ppm amahora angu-24.

Sincoma u-2 g we-subsieve kumabhodlela e-reagent angu-50-250ml, u-5g kumabhodlela e-reagent angu-250-500ml, u-10g kumabhodlela anyakazayo angu-500-1000ml, kanye no-20g kumabhodlela anyakazayo angu-1000-2000ml.

Isiqiniseko

Iphakethe ngalinye lesikrini esincane lilawulwa ngokuqinile ikhwalithi phakathi nenqubo yokukhiqiza.Futhi kuyadingeka ukuthi amakhasimende ahlole ukupakishwa ngaphambi kokusetshenziswa:

Okokuqala, qinisekisa ukuthi i-vacuum yokupakisha iphelele.Noma yikuphi ukuvuza kwe-vacuum noma ukungena komoya kuzonciphisa ukusebenza komkhiqizo.

Okwesibili, qaphela lapho uvula okokufaka ukuze uvimbele ifilimu yokupakisha yesikrini esingaphansi ukuthi ingaklwebheki, futhi uhlole ukuthi ingabe ifilimu ikhona Kukhona ukuvuza.

Ukwelashwa kwangemuva kokusetshenziswa

Ayinabuthi noma iyingozi, izongcoliswa amakhemikhali nezinyibilikisi ezihlangana nazo futhi kufanele zilahlwe njengemfucuza engcolile ngemva kokusetshenziswa.

Imodeli kanye nesicelo

Ngaphambilini sinikeze izikhwama ze-sub-sieve ezinosayizi ongu-2 g, 5 g, 10 g, no-20 g., kanye nokunye okucacisiwe kungenziwa ngendlela oyifisayo ngokwezidingo zamakhasimende.

Isicupho samangqamuzana silungele ukuchayeka isikhathi eside ku-nitrile, futhi singase sisetshenziselwe i-acetic acid, i-ether, i-acetic ether, i-butyl acetate, utshwala, isopropanol, i-methanol, i-butanol, i-Phenol, i-pyridine, i-hydrochloric acid, i-nitric acid, i-phosphoric acid. , i-sulfuric acid, i-methyl chloride, i-nitrogen methyl imidazole, njll.

Kungase kungafaneleki ukuchayeka isikhathi eside ku-tetrahydrofuran, toluene, methyl formamide (DMF), i-methyl methyl amide (DMAc), i-N-methylpyrrolidone (NMP) nezinye izixazululo.

Izicupho zamangqamuzana3
Izicupho zamangqamuzana4
Izicupho ze-Molecular5

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